Journal: PLoS ONE

Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

doi: 10.1371/journal.pone.0148075

Figure Lengend Snippet: (A) MMP1 secretion level of stable MDCK- MMP1 colonies. (B) Cellular adhesion quantified by shear flow chamber assays. Significance: ** p-value < 0.03, * p-value > 0.05.

Article Snippet: The amplified vector pOTB7-MMP1 was digested using StuI and XhoI enzymes (New England Biolabs) at 37°C for 2 h to release the gene insert.

Techniques:

Journal: PLoS ONE

Article Title: Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells

doi: 10.1371/journal.pone.0148075

Figure Lengend Snippet: Diagrams are simplified based on known mechanisms. (A) Hyposialyation leads to diminished response of the receptor and thus no pathway activation. (B) Sufficient sialylation on MET allows activation by its ligand, HGF, causing up-regulation of MMP1 and down-regulation of cell adhesion molecules.

Article Snippet: The amplified vector pOTB7-MMP1 was digested using StuI and XhoI enzymes (New England Biolabs) at 37°C for 2 h to release the gene insert.

Techniques: Activation Assay